E-ISSN 2146-3077
Original Article
Diagnosis of Trichomoniasis in Male Patients on Performing Nested Polymerase Chain Reaction
1 Department of Parasitology, Turgut Özal Medical Center, İnönü University Faculty of Medicine Malatya, Turkey  
2 Department of Parasitology, Ege University Faculty of Medicine, İzmir, Turkey  
3 Department of Biochemistry, Turgut Özal Medical Center, İnönü University Faculty of Medicine Malatya, Turkey  
Turkiye Parazitol Derg 2017; 41: 130-134
DOI: 10.5152/tpd.2017.5016
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Key Words: Trichomonas vaginalis, direct microscopy, culture, nested PCR
Abstract

Objective: Trichomoniasis is a parasitic infection that occurs with the settlement of Trichomonas vaginalis in female and male urinary and reproductive tracts. This infection is generally asymptomatic in males, and males are thought to be a carrier for the transmission of infection. In this study, our aim was to detect trichomoniasis using nested polymerase chain reaction among males who were referred to a hospital with suspected urinary tract infection.

 

Methods: Urine samples were collected from 138 male patients between 18 and 50 years of age who were referred with suspected urinary system infection to the Urology Outpatient Clinic at Malatya University Medical Center Malatya between December 2013 and May 2014. Direct microscopy, two different culture methods, and nested Polymerase chain reaction (PCR) were performed for the investigation of T. vaginalis in urine samples.

 

Results: Urinary tract infection was diagnosed in 47 of the 138 patients according to white and red blood cell counts in the urine samples. T. vaginalis infection was detected in 6.5% (9/138) of the suspected patients by nested PCR, while none of the samples tested positive by direct microscopy and culture examinations. Statistical significance was found between infection of the urinary tract and nested PCR positivity for T. vaginalis.

 

 

Conclusions: According to our results, nested PCR is the most sensitive method for the detection of trichomoniasis in male patients. We strongly recommend using nested PCR for the differential diagnosis of urinary infections in males.

 

Cite this article as: Yar TM, Karakuş M, Töz S, Bay Karabulut A, Özbel Y, Atambay M. Diagnosis of Trichomoniasis in Male Patients on Performing Nested Polymerase Chain Reaction. Türkiye Parazitol Derg 2017; 41: 130-4.

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