E-ISSN 2146-3077
Original Article
Effect of Extracts of the Endemic Plants Centaurea lydia and Phlomis nissolii on Toxoplasma gondii
1 Ege Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İzmir, Türkiye  
2 İzmir Büyükçiğli Özel Türk Koleji, İzmir, Türkiye  
3 İzmir Katip Çelebi Üniversitesi Orman Fakültesi, Orman Botaniği Anabilim Dalı, İzmir, Türkiye  
4 Ege Üniversitesi Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, İzmir, Türkiye  
5 Celal Bayar Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, Manisa, Türkiye  
6 Ege Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, İzmir, Türkiye  
Turkiye Parazitol Derg 2017; 41: 164-168
DOI: 10.5152/tpd.2017.5451
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Key Words: Toxoplasma gondii, Centaurea lydia, Phlomis nissolii, cytotoxicity, cell culture
Abstract

Objective: This study aimed to investigate the potential antitoxoplasma activities of extracts of the endemic plants Centaurea lydia and Phlomis nissolii in a fibroblast cell culture infected with T. gondii trophozoites.

 

Methods: WI-38 cell lines treated with plant extracts (55 μg/mL each) and an untreated control were infected with 5×105 T. gondii trophozoites, and the number of parasites in the medium was determined on days 7, 14, and 24.

 

Results: No cytotoxic effects of C. lydia and P. nissolii extracts were detected at concentrations of 0.86–55 μg/mL in the WI-38 cell line, and the absence of the cytotoxicity of these extracts on the fibroblast cell line was considered as a positive effect. C. lydia extract at 55 μg/mL had marked activity against T. gondii trophozoites. A 47.5-fold increase was observed in the number of trophozoites in the control group, while a 84-fold decrease was found in the C. lydia extract group. However, a 36-fold increase was detected in the P. nissolii extract group, indicating no antitoxoplasma activity.

 

 

Conclusion: The extract of C. lydia, an endemic plant, was found to be a good drug candidate for treating toxoplasmosis. The in vitro activity of the extract of this endemic plant should be further investigated in animal models in vivo.

 

Cite this article as: Doğan Şığva ZÖ, Hasvatan EE, Gülen G, Uslu R, Eryıldız B, Durmuşkaya C, Kayalar H, Özbilgin A, Korkmaz M, Gündüz C. Effect of Extracts of the Endemic Plants Centaurea lydia and Phlomis nissolii on Toxoplasma gondii. Türkiye Parazitol Derg 2017; 41: 164-8.

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