E-ISSN 2146-3077
Original Article
Detection of Cryptosporidium spp. in Feces with Nested PCR and Carbol Fuchsin Staining Method
1 Gazi Üniversitesi Fen Fakültesi, Biyoloji Anabilim Dalı, Ankara  
2 Namık Kemal Üniversitesi, Fen‐Edebiyat Fakültesi, Biyoloji Bölümü, Tekirdağ, Türkiye  
3 Atatürk Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Erzurum, Türkiye  
4 Gazi Üniversitesi Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Ankara, Türkiye  
5 Ankara Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Ankara, Türkiye  
6 Kafkas Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Kars, Türkiye  
Turkiye Parazitol Derg 2008; 32: 305-308

Key Words: Cryptosporidiosis, nested PCR, carbol fuchsin, human, calves
Abstract

This study was carried out on 45 stool specimens, consisting of 18 samples from children with diarrhea and 27 samples from diarrheic calves. Samples were screened by both carbol fuchsin staining and nested PCR for presence of Cryptosporidium spp. Using the carbol fuchsin staining method, we detected a total of 4 (8.9%) positive samples out of 45; of these 3 (%11,2) were from calf samples and 1 (5.6%) from a child. Nested PCR detected a total of 9 (20.0% positive samples out of 45 including 8 (29.7%) from calf samples and 1 (5.6%) from a child. Although the staining method revealed a 100% specificity, it was deficient in sensitivity (44.0%) compared to nested PCR. The study showed that nested PCR is an acceptable method for studying the etiology of doubtful diarrheal cases. 

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