E-ISSN 2146-3077
Original Article
Detection of Entamoeba histolytica in Stool Specimens with the ELISA Method
1 Zonguldak Karaelmas Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Zonguldak, Türkiye  
Turkiye Parazitol Derg 2009; 33: 1-3

Key Words: Amebic cyst, Entamoeba, ELISA
Abstract

In this study, carried out between November 2006 and September 2007, amebic cysts were observed in 44 (0.37%) out of a total of 1720 stool specimens which were examined by direct microscopy. Entamoeba histolytica specific antigen was investigated with ELISA in the specimens in which cysts were observed. Specific antigen was detected in 26 (59.1%) of these specimens. The diagnosis of amoebiasis for the patients whose ELISA tests were positive was confirmed and the appropriate therapy was immediately begun. This prediagnosis was abandoned for patients whose test results were “negative”. Because of the low sensitivity of direct microscopy in confirming the prediagnosis of amoebiasis, it is necessary to perform ELISA on the specimens in order to determine whether the patient should be treated or to prevent patients from being given an unnecessary treatment. 

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