E-ISSN 2146-3077
Original Article
Molecular Characterization and Chronobiology of Hypodermosis in Cattle Slaughtered in the Diyarbakir Province of Turkey
1 Department of Parasitology, Dicle University School of Veterinary, Diyarbakır, Turkey  
Turkiye Parazitol Derg 2016; 40: 86-89
DOI: 10.5152/tpd.2016.4812
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Key Words: Hypoderma bovis, chronobiology, PCR-RFLP, cattle, Diyarbakir
Abstract

Objective: The aim of present study was to investigate the chronobiology and identification of Hypoderma species in cattle slaughtered in the Diyarbakir Province of Turkey.

 

Methods: In total, 736 hides and subcutaneous tissue of slaughtered cattle were examined for the presence of second- and third-instar larvae in the slaughterhouse between November 2012 and May 2013. Third-instar larvae were collected from the slaughterhouses, and gDNA isolates were examined by PCR-RFLP analysis of the cytochrome c oxidase I (COI) gene of mt-DNA using TaqI enzyme.

 

Results: In total, 62 out of 736 cattle (8.42%) were found to be positive for Hypoderma larvae. A total of 328 (90 second- and 238 third-instar) Hypoderma larvae were detected in the hide and subcutaneous tissue of the back of infested cattle. All the 238 third-instar larvae (100%) were identified as H. bovis by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The mean number of Hypoderma larvae in each cow was 5.29% (62/736). In the examined cattle, second-instar larvae were observed starting from the second week of January and subcutaneous nodules were found until the last week of April.

 

Conclusion: H. bovis was the dominant species detected in the Diyarbakir Province. 

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